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验证数据展示

  • WB analysis of HepG2 using 84685-3-RR

    WB analysis of HepG2 using 84685-3-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84685-3-RR (Serpin F1/PEDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of C2C12 using 84685-3-RR

    WB analysis of C2C12 using 84685-3-RR

    C2C12 cells were subjected to SDS PAGE followed by western blot with 84685-3-RR (Serpin F1/PEDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 84685-3-RR

    FC experiment of HEK-293 using 84685-3-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Serpin F1/PEDF Recombinant antibody (84685-3-RR, Clone:242127F9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84685-3-RR

    Affinity and Kinetic Characterization of 84685-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84685-3-RR against Human Serpin F1/PEDF were performed. The affinity constant is 1.08 nM.

Serpin F1/PEDF Recombinant antibody

Serpin F1/PEDF Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84685-3-RR

产品说明书

CloneNo. 242127F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

PEDF, PIG35, 242127F9, Cell proliferation-inducing gene 35 protein, EPC 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, C2C12 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy SERPINF1/PEDF Ready-To-Use IHC Kit. SERPINF1/PEDF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84685-3-RR targets Serpin F1/PEDF in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
别名 PEDF, PIG35, 242127F9, Cell proliferation-inducing gene 35 protein, EPC 1
计算分子量46kDa
观测分子量 46 kDa
GenBank蛋白编号NM_002615.7
基因名称 PEDF
Gene ID (NCBI) 5176
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP36955
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.

实验方案

Product Specific Protocols
WB protocol for Serpin F1/PEDF antibody 84685-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols