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验证数据展示

  • WB analysis using 19559-1-AP (same clone as 19559-1-PBS)

    WB analysis using 19559-1-AP (same clone as 19559-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19559-1-AP (Serotonin transporter antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 19559-1-AP (same clone as 19559-1-PBS)

    IF Staining of mouse cerebellum using 19559-1-AP (same clone as 19559-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Serotonin transporter antibody (19559-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

  • IF Staining of rat cerebellum using 19559-1-AP (same clone as 19559-1-PBS)

    IF Staining of rat cerebellum using 19559-1-AP (same clone as 19559-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Serotonin transporter antibody (19559-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Serotonin transporter Polyclonal antibody, PBS Only

Serotonin transporter Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 19559-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, Indirect ELISA

别名

SLC6A4, 5 HTT, 5 HTTLPR, 5HTT, hSERT

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

19559-1-PBS targets Serotonin transporter in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
别名 SLC6A4, 5 HTT, 5 HTTLPR, 5HTT, hSERT
计算分子量 70 kDa
观测分子量70 kDa and 120 kDa
GenBank蛋白编号NM_001045
基因名称 Serotonin transporter
Gene ID (NCBI) 6532
RRIDAB_2878590
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31645
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or via movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, in part by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319). This antibody detected 70 kDa and 120 kDa bands in SDS-PAGE. The 120 kDa band corresponds to the glycosylation form of SERT (PMID: 11311448).

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