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验证数据展示

  • WB analysis of human plasma using 30249-1-AP (same clone as 30249-1-PBS)

    WB analysis of human plasma using 30249-1-AP (same clone as 30249-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 30249-1-AP (Selenoprotein P antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30249-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 30249-1-AP (same clone as 30249-1-PBS)

    IHC staining of human liver using 30249-1-AP (same clone as 30249-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30249-1-AP (Selenoprotein P antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30249-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 30249-1-AP (same clone as 30249-1-PBS)

    IF Staining of HepG2 using 30249-1-AP (same clone as 30249-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Selenoprotein P antibody (30249-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30249-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 30249-1-AP (same clone as 30249-1-PBS)

    FC experiment of HepG2 using 30249-1-AP (same clone as 30249-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Selenoprotein P Polyclonal antibody (30249-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 30249-1-PBS in a different storage buffer formulation.

Selenoprotein P Polyclonal antibody, PBS Only

Selenoprotein P Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 30249-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

SEPP1, SELENOP

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30249-1-PBS targets Selenoprotein P in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32952

Product name: Recombinant human Selenoprotein P protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 82-299 aa of NM_001085486.2

Sequence: SNISYIVVNHQGISSRLKYTHLKNKVSEHIPVYQQEENQTDVWTLLNGSKDDFLIYDRCGRLVYHLGLPFSFLTFPYVEEAIKIAYCEKKCGNCSLTTLKDEDFCKRVSLATVDKTVETPSPHYHHEHHHNHGHQHLGSSELSENQQPGAPNAPTHPAPPGLHHHHKHKGQHRQGHPENRDMPASEDLQDLQKKLCRKRCINQLLCKLPTDSELAPRS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 selenoprotein P, plasma, 1
别名 SEPP1, SELENOP
计算分子量43 kDa
观测分子量 45-50 kDa
GenBank蛋白编号NM_001085486.2
基因名称 Selenoprotein P
Gene ID (NCBI) 6414
RRIDAB_3086280
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49908
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Selenoprotein P, also known as SEPP1, is expressed in the liver and heart and secreted into the plasma(PMID: 8421687). Selenoprotein P is implicated as an extracellular antioxidant involved in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2) and provides selenium to tissues such as brain and testis. In humans, it exists in plasma as two isoforms of approximately 50 and 60 kDa(PMID:19453253). Selenoprotein P is a glycosylated protein and deglycosylation of SEPP1 results in 45 kDa and 35 kDa isoforms(PMID: 25298198).

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