验证数据展示

WB analysis using 28679-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 28679-1-AP (SAMM50 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 28679-1-AP
WB result of Sam50 antibody (28679-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Sam50 transfected A431 cells.
IF Staining of HeLa using 28679-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (28679-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IHC staining of human heart using 28679-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SAMM50 antibody included
IHC staining of human heart using 28679-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SAMM50 antibody included
IHC staining of mouse colon using 28679-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SAMM50 antibody included
IHC staining of human ovary cancer using 28679-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SAMM50 antibody included
IHC staining of human placenta using 28679-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SAMM50 antibody included
IHC staining of mouse adrenal gland using 28679-1-AP
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SAMM50 antibody included
IHC staining of mouse kidney using 28679-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SAMM50 antibody included
IHC staining of rat adrenal gland using 28679-1-AP
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SAMM50 antibody included
IHC staining of rat colon using 28679-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SAMM50 antibody included
IHC staining of rat kidney using 28679-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SAMM50 antibody included

Featured Product
KD/KO Validated

Sam50 Polyclonal antibody

Sam50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 28679-1-AP

产品说明书

引用文献(11)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

SAMM50, CGI 51, CGI-51, OMP85, SAMM 50

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (11)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A431 cells, HeLa cells, HepG2 cells, mouse heart tissur, rat heart tissue
Positive IHC detected in	human heart tissue, human liver cancer tissue, mouse adrenal gland tissue, rat kidney tissue, rat adrenal gland tissue, human placenta tissue, mouse kidney tissue, human ovary cancer tissue, mouse colon tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy® SAMM50 Ready-To-Use IHC Kit. SAMM50 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 8 publications below
IF	See 2 publications below
IP	See 1 publications below

产品信息

28679-1-AP targets Sam50 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, ELISA Application Description
文献引用应用	WB, IF, IP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag29152 Product name: Recombinant human SAMM50 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC007830 Sequence: MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPW 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	sorting and assembly machinery component 50 homolog (S. cerevisiae)
别名	SAMM50, CGI 51, CGI-51, OMP85, SAMM 50
计算分子量	469 aa, 52 kDa
观测分子量	52 kDa
GenBank蛋白编号	BC007830
基因名称	Sam50
Gene ID (NCBI)	25813
RRID	AB_2881192
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9Y512
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

实验方案

Product Specific Protocols
IF protocol for Sam50 antibody 28679-1-AP	Download protocol
IHC protocol for Sam50 antibody 28679-1-AP	Download protocol
WB protocol for Sam50 antibody 28679-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
rat,mouse,human	WB	J Nanobiotechnology UBQLN1 Inhibition reduces MASH progression through downregulating SIKE/p38 MAPK pathway in hepatocyte. Authors - Yifei Chen View Article
human	WB	Brain Mitochondrial damage is associated with an early immune response in inclusion body myositis. Authors - D Felix Kleefeld View Article
mouse	WB	Int J Biol Sci Loss of MTX2 causes mitochondrial dysfunction, podocyte injury, nephrotic proteinuria and glomerulopathy in mice and patients Authors - Ting Li View Article
mouse,human	WB	Dev Cell Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation Authors - Wanxin Guo View Article
mouse	WB	Cells Heart-Specific and Conditional Deletion of the Immt Gene Reveals Its Role in Regulating Mitochondrial Structure and Total Heart Metabolism. Authors - Yasuhide Kuwabara View Article
		Biol Proced Online High Expression of SAMM50 Indicates Poor Clinical Prognosis in Hepatocellular Carcinoma and Represents a Potential Novel Biomarker. Authors - Huawei Zhai View Article

验证数据展示

WB analysis using 28679-1-AP

WB analysis of A431 using 28679-1-AP

IF Staining of HeLa using 28679-1-AP

IHC staining of human heart using 28679-1-AP

IHC staining of human heart using 28679-1-AP

IHC staining of mouse colon using 28679-1-AP

IHC staining of human ovary cancer using 28679-1-AP

IHC staining of human placenta using 28679-1-AP

IHC staining of mouse adrenal gland using 28679-1-AP

IHC staining of mouse kidney using 28679-1-AP

IHC staining of rat adrenal gland using 28679-1-AP

IHC staining of rat colon using 28679-1-AP

IHC staining of rat kidney using 28679-1-AP

Sam50 Polyclonal antibody

Sam50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

UBQLN1 Inhibition reduces MASH progression through downregulating SIKE/p38 MAPK pathway in hepatocyte.

Mitochondrial damage is associated with an early immune response in inclusion body myositis.

Loss of MTX2 causes mitochondrial dysfunction, podocyte injury, nephrotic proteinuria and glomerulopathy in mice and patients

Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation

Heart-Specific and Conditional Deletion of the Immt Gene Reveals Its Role in Regulating Mitochondrial Structure and Total Heart Metabolism.

High Expression of SAMM50 Indicates Poor Clinical Prognosis in Hepatocellular Carcinoma and Represents a Potential Novel Biomarker.

Able™

Able^™