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验证数据展示

  • WB analysis using 12793-1-AP

    WB analysis using 12793-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12793-1-AP (Synaptotagmin-9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Synaptotagmin-9 Polyclonal antibody

Synaptotagmin-9 Polyclonal Antibody for WB, ELISA
Cat No. 12793-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Synaptotagmin IX, SYT9, SytIX

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse eye tissue, human brain tissue, human kidney tissue, rat eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12793-1-AP targets Synaptotagmin-9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3520

Product name: Recombinant human SYT9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 45-319 aa of BC029605

Sequence: LCWVPWRERGLPSGSKDNNQEPLNYMDTETNEQENSEDFLDPPTPCPDSSMKISHTSPDIPLSTQTGIQENCAHGVRVQRQVTEPTSSARHNSIRRQLNLSNPDFNIQQLQKQEQLTGIGRIKPELYKQRSLDNDDGRRSNSKACGKLNFILKYDCDLEQLIVKIHKAVNLPAKDFSGTSDPYVKIYLLPDRKTKHQTKVHRKTLNPVFDEVFLFPVPYNDLEARKLHFSVYDFDRFSRHDLIGQVVVDHFLDLADFPRECILWKDIEYVTNILR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptotagmin IX
别名 Synaptotagmin IX, SYT9, SytIX
计算分子量 491 aa, 52 kDa
观测分子量 52-56 kDa
GenBank蛋白编号BC029605
基因名称 Synaptotagmin-9
Gene ID (NCBI) 143425
RRIDAB_2255659
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86SS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-9 (SYT9) is a tandem C2 domain Ca 2+ sensor for exocytosis in neuroendocrine cells (PMID: 36732068).

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-9 antibody 12793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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