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验证数据展示

  • WB analysis using 19741-1-AP

    WB analysis using 19741-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19741-1-AP (SYT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 19741-1-AP

    IF Staining of mouse cerebellum using 19741-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT7 antibody (19741-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 19741-1-AP

    IHC staining of mouse brain using 19741-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19741-1-AP (SYT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 19741-1-AP

    IHC staining of mouse cerebellum using 19741-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19741-1-AP (SYT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SYT7 Polyclonal antibody

SYT7 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 19741-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA and More (1)

别名

IPCA 7, IPCA-7, PCANAP7, Prostate cancer-associated protein 7, Synaptotagmin 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

19741-1-AP targets SYT7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptotagmin VII
别名 IPCA 7, IPCA-7, PCANAP7, Prostate cancer-associated protein 7, Synaptotagmin 7
计算分子量 46 kDa
观测分子量42-45 kDa
GenBank蛋白编号NM_004200
基因名称 SYT7
Gene ID (NCBI) 9066
RRIDAB_3085583
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43581
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYT7, also named as PCANAP7 and IPCA-7, belongs to the synaptotagmin family. SYT7 may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

实验方案

Product Specific Protocols
WB protocol for SYT7 antibody 19741-1-APDownload protocol
IHC protocol for SYT7 antibody 19741-1-APDownload protocol
IF protocol for SYT7 antibody 19741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Brain Res Bull

Double-strand-break repair protein rad21 homolog/Synaptotagmin-7 alleviates Alzheimer's disease in mice by promoting M2 polarization of microglia

Authors - Cheng Zhu
View Article