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SYT7 Polyclonal antibody

SYT7 Polyclonal Antibody for IF-P, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, WB, ELISA

Conjugate

Unconjugated

Cat No : 19741-1-AP

Print datasheet

Synonyms

IPCA 7, PCANAP7, Synaptotagmin 7, synaptotagmin VII, SYT VII, SYT7, SytVII

验证数据展示

  • WB analysis using 19741-1-AP

    WB analysis using 19741-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19741-1-AP (SYT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 19741-1-AP

    IF Staining of mouse cerebellum using 19741-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT7 antibody (19741-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IF detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19741-1-AP targets SYT7 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, WB, ELISA
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptotagmin VII
Synonyms IPCA 7, PCANAP7, Synaptotagmin 7, synaptotagmin VII, SYT VII, SYT7, SytVII
Calculated Molecular Weight 46 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberNM_004200
Gene Symbol SYT7
Gene ID (NCBI) 9066
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43581
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYT7, also named as PCANAP7 and IPCA-7, belongs to the synaptotagmin family. SYT7 may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

实验方案

Product Specific Protocols
WB protocol for SYT7 antibody 19741-1-APDownload protocol
IF protocol for SYT7 antibody 19741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols