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验证数据展示

  • WB analysis using 17488-1-AP

    WB analysis using 17488-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17488-1-AP (Synaptotagmin-6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17488-1-AP

    IHC staining of human liver using 17488-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17488-1-AP (Synaptotagmin-6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 17488-1-AP

    IHC staining of human liver using 17488-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17488-1-AP (SYT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Synaptotagmin-6 Polyclonal antibody

Synaptotagmin-6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17488-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Synaptotagmin 6, synaptotagmin VI, SYT6, SytVI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17488-1-AP targets Synaptotagmin-6 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Synaptotagmin-6 fusion protein Ag11575 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptotagmin VI
别名 Synaptotagmin 6, synaptotagmin VI, SYT6, SytVI
计算分子量425aa,48 kDa; 510aa,57 kDa
观测分子量70 kDa
GenBank蛋白编号BC044948
基因名称 Synaptotagmin-6
Gene ID (NCBI) 148281
RRIDAB_3085525
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T7P8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-6 antibody 17488-1-APDownload protocol
IHC protocol for Synaptotagmin-6 antibody 17488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols