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SYT2 Polyclonal antibody

SYT2 Polyclonal Antibody for IF-P, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, WB, ELISA

Conjugate

Unconjugated

Cat No : 21245-1-AP

Print datasheet

Synonyms

Synaptotagmin 2, synaptotagmin II, SYT2, SytII

验证数据展示

  • WB analysis using 21245-1-AP

    WB analysis using 21245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21245-1-AP (SYT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 21245-1-AP

    IF Staining of mouse cerebellum using 21245-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT2 antibody (21245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 21245-1-AP

    IF Staining of mouse cerebellum using 21245-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT2 antibody (21245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, Neuro-2a cells
Positive IF detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21245-1-AP targets SYT2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SYT2 fusion protein Ag15744 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptotagmin II
Synonyms Synaptotagmin 2, synaptotagmin II, SYT2, SytII
Calculated Molecular Weight 419 aa, 47 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC100815
Gene Symbol SYT2
Gene ID (NCBI) 127833
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9I0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYT2 (Synaptotagmin-2) a synaptic vesicle membrane protein, functions as a calcium sensor in vesicular trafficking and exocytosis. SYT2 is also plays a role in dendrite formation by melanocytes (PMID: 23999003). Mutations in SYT2 are associated with myasthenic syndrome, presynaptic, congenital, with or without motor neuropathy. SYT2 mutations cause a complex presynaptic congenital myasthenic syndrome (PMID: 26519543).

实验方案

Product Specific Protocols
WB protocol for SYT2 antibody 21245-1-APDownload protocol
IF protocol for SYT2 antibody 21245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols