验证数据展示
经过测试的应用
| Positive WB detected in | human kidney tissue, A375 cells, human brain tissue, human testis tissue, mouse brain tissue, mouse lung tissue, PC-3 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
15413-1-AP targets SYT17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | SYT17 fusion protein Ag7691 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | synaptotagmin XVII |
| 别名 | synaptotagminXVII, Synaptotagmin-17, Synaptotagmin17, Synaptotagmin XVII, Synaptotagmin 17 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 75-80 kDa |
| GenBank蛋白编号 | BC004518 |
| 基因名称 | SYT17 |
| Gene ID (NCBI) | 51760 |
| RRID | AB_2199457 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BSW7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SYT17, or synaptotagmin 17, is a protein that belongs to the synaptotagmin family, which is known for its role in regulating exocytosis at the plasma membrane. Reports find high levels of expression in hippocampus, particularly in pyramidal cell layers and in cultured hippocampal neurons. SYT17 is thus a multifunctional regulator of intracellular protein trafficking in excitatory hippocampal neurons, modulating both neural development and synaptic physiology. (PMID: 31387992, PMID: 36332665)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SYT17 antibody 15413-1-AP | Download protocol |
| IHC protocol for SYT17 antibody 15413-1-AP | Download protocol |
| IF protocol for SYT17 antibody 15413-1-AP | Download protocol |
| IP protocol for SYT17 antibody 15413-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
