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Synaptotagmin-11 Polyclonal antibody

Synaptotagmin-11 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12031-1-AP

Print datasheet

Synonyms

KIAA0080, Synaptotagmin 11, synaptotagmin XI, Synaptotagmin-11, SYT11, SytXI

验证数据展示

  • WB analysis using 12031-1-AP

    WB analysis using 12031-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12031-1-AP (Synaptotagmin-11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 12031-1-AP

    IHC staining of mouse brain using 12031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12031-1-AP (Synaptotagmin-11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 12031-1-AP

    IHC staining of mouse brain using 12031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12031-1-AP (Synaptotagmin-11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat brain tissue, mouse skeletal muscle tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12031-1-AP targets Synaptotagmin-11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen Synaptotagmin-11 fusion protein Ag2663 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptotagmin XI
Synonyms KIAA0080, Synaptotagmin 11, synaptotagmin XI, Synaptotagmin-11, SYT11, SytXI
Calculated Molecular Weight 431 aa, 48 kDa
Observed Molecular Weight 48 kDa，96 kDa
GenBank Accession NumberBC013690
Gene Symbol SYT11
Gene ID (NCBI) 23208
RRIDAB_10638432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BT88
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-11 antibody 12031-1-APDownload protocol
IHC protocol for Synaptotagmin-11 antibody 12031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols