HCT 116 (WT and SYT1 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 14511-1-AP incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

mouse cerebellum tissue was subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

HCT 116 lysates prepared and IP of SYT1 performed using 1.0 μg of 14511-1-AP coupled to protein A- Sepharose beads. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

HCT 116 WT cells (yellow outline) and SYT1 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 14511-1-AP at 1:500 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

IP result of anti-Synaptotagmin-1 (IP:14511-1-AP, 4ug; Detection:14511-1-AP 1:20000) with mouse cerebellum tissue lysate 2000 ug.

Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).