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验证数据展示

  • WB analysis using RMX00068

    WB analysis using RMX00068

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00068 (ARIP2; SYNJ2BP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis using RMX00068

    WB analysis using RMX00068

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00068 (ARIP2; SYNJ2BP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using RMX00068

    IHC staining of mouse liver using RMX00068

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00068 (ARIP2; SYNJ2BP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using RMX00068

    IF Staining of HepG2 using RMX00068

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARIP2; SYNJ2BP antibody (RMX00068) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Multi-rAb® SYNJ2BP Multi-Recombinant antibody

SYNJ2BP Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00068

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ARIP2, OMP25, Synaptojanin-2-binding protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, mouse kidney tissue, HEK-293 cells, SGC-7901 cells, mouse liver tissue, rat kidney tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00068 targets SYNJ2BP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 synaptojanin 2 binding protein
别名 ARIP2, OMP25, Synaptojanin-2-binding protein
计算分子量 145 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC007704
基因名称 SYNJ2BP
Gene ID (NCBI) 55333
RRIDAB_3750117
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP57105
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. SYNJ2BP is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. SYNJ2BP may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. (PMID: 23284606).

实验方案

Product Specific Protocols
IF protocol for SYNJ2BP antibody RMX00068Download protocol
WB protocol for SYNJ2BP antibody RMX00068Download protocol
IHC protocol for SYNJ2BP antibody RMX00068Download protocol
Standard Protocols
Click here to view our Standard Protocols
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