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验证数据展示

  • IP experiment of mouse heart using 25151-1-AP

    IP experiment of mouse heart using 25151-1-AP

    IP result of anti-Syncoilin (IP:25151-1-AP, 4ug; Detection:25151-1-AP 1:800) with mouse heart tissue lysate 4000ug.

  • IHC staining of human skeletal muscle using 25151-1-AP

    IHC staining of human skeletal muscle using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25151-1-AP (SYNC Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 25151-1-AP

    IHC staining of human skeletal muscle using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25151-1-AP (SYNC Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 25151-1-AP

    WB analysis using 25151-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25151-1-AP (Syncoilin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 25151-1-AP

    IHC staining of mouse heart using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 25151-1-AP

    IHC staining of mouse heart using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 25151-1-AP

    IHC staining of mouse skeletal muscle using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 25151-1-AP

    IHC staining of mouse skeletal muscle using 25151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Syncoilin Polyclonal antibody

Syncoilin Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 25151-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA and More (1)

别名

SYNC, Syncoilin-1, Syncoilin 1, SYNC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

25151-1-AP targets Syncoilin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 Syncoilin fusion protein Ag18565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 syncoilin, intermediate filament protein
别名 SYNC, Syncoilin-1, Syncoilin 1, SYNC1
计算分子量 482 aa, 55 kDa
观测分子量 64-68 kDa, 55 kDa
GenBank蛋白编号BC119700
基因名称 SYNC
Gene ID (NCBI) 81493
RRIDAB_2879926
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7C4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syncoilin also known as SYNC1, is a member of the intermediate filament family with an N-terminal head domain, followed by a central coiled-coil region and a short C-terminal tail. Syncoilin is highly expressed in skeletal and cardiac muscle. In normal skeletal muscle, syncoilin is concentrated at the neuromuscular junction(PMID: 11053421). Western blot analysis detected 64 kDa and 55 kDa syncoilin proteins in mouse heart(PMID: 11053421).

实验方案

Product Specific Protocols
WB protocol for Syncoilin antibody 25151-1-APDownload protocol
IHC protocol for Syncoilin antibody 25151-1-APDownload protocol
IP protocol for Syncoilin antibody 25151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Invest Ophthalmol Vis Sci

The Cytoskeleton in the Extraocular Muscles of Desmin Knockout Mice.

Authors - Maria Angels Rodríguez
View Article