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验证数据展示

  • WB analysis of K-562 using 11519-1-AP

    WB analysis of K-562 using 11519-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11519-1-AP (Symplekin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 11519-1-AP

    IHC staining of human testis using 11519-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SYMPK antibody included
  • IHC staining of human testis using 11519-1-AP

    IHC staining of human testis using 11519-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with SYMPK antibody included
  • IP experiment of K-562 using 11519-1-AP

    IP experiment of K-562 using 11519-1-AP

    IP result of anti-Symplekin (IP:11519-1-AP, 4ug; Detection:11519-1-AP 1:500) with K-562 cells lysate 3600ug.

Symplekin Polyclonal antibody

Symplekin Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11519-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

别名

SPK, SYM, SYMPK, symplekin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman testis tissue, human colon cancer tissue, human liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SYMPK Ready-To-Use IHC Kit. SYMPK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11519-1-AP targets Symplekin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 Symplekin fusion protein Ag2081 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 symplekin
别名 SPK, SYM, SYMPK, symplekin
计算分子量 1274 aa, 141 kDa
观测分子量 150 kDa
GenBank蛋白编号BC006567
基因名称 Symplekin
Gene ID (NCBI) 8189
RRIDAB_2200281
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92797
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Symplekin antibody 11519-1-APDownload protocol
IHC protocol for Symplekin antibody 11519-1-APDownload protocol
IP protocol for Symplekin antibody 11519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Front Cell Dev Biol

SYMPK Is Required for Meiosis and Involved in Alternative Splicing in Male Germ Cells

Authors - Rui Wu
  • KO Validated
View Article