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验证数据展示

  • WB analysis using 86648-2-RR

    WB analysis using 86648-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86648-2-RR (SYCP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 86648-2-RR

    IHC staining of human testis using 86648-2-RR

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 86648-2-RR (SYCP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 86648-2-RR

    Affinity of 86648-2-RR

    Biolayer interferometry (BLI) kinetic assays of 86648-2-RR against Human SYCP3 were performed. The affinity constant is 0.56 nM.

SYCP3 Recombinant monoclonal antibody

SYCP3 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86648-2-RR

产品说明书

CloneNo. 251653A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SCP3, COR1, SCP 3, SCP-3, Synaptonemal complex protein 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86648-2-RR targets SYCP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32466

Product name: Recombinant human SYCP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 137-236 aa of BC062662

Sequence: DLDMQKAEEQEEKILNMFRQQQKILQQSRIVQSQRLKTIKQLYEQFIKSMEELEKNHDNLLTGAQNEFKKEMAMLQKKIMMETQQQEIASVRKSLQSMLF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptonemal complex protein 3
别名 SCP3, COR1, SCP 3, SCP-3, Synaptonemal complex protein 3
计算分子量 236 aa, 28 kDa
观测分子量30 kDa, 33 kDa
GenBank蛋白编号BC062662
基因名称 SYCP3
Gene ID (NCBI) 50511
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IZU3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYCP3 is an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. SYCP3 is required for centromere pairing during meiosis in male germ cells. SYCP3 is also required for normal meiosis during spermatogenesis and male fertility. Mutations in SYCP3 are associated with azoospermia in males and susceptibility to pregnancy loss in females.

实验方案

Product Specific Protocols
IHC protocol for SYCP3 antibody 86648-2-RRDownload protocol
WB protocol for SYCP3 antibody 86648-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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