验证数据展示
经过测试的应用
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16859-1-AP targets SYCE2-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | synaptonemal complex central element protein 2 |
| 别名 | SYCE2, CESC1 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | NM_001105578 |
| 基因名称 | SYCE2 |
| Gene ID (NCBI) | 256126 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6PIF2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SYCE2 (synaptonemal complex central element protein 2) is a structural component of the synaptonemal complex (SC) that is essential for meiotic chromosome synapsis and homologous recombination during meiosis. SYCE2 localizes to the central element of the synaptonemal complex, where it interacts with other SC proteins, including SYCE1, SYCE3, and TEX12, to promote assembly and stabilization of the central region. Through these interactions, SYCE2 facilitates homologous chromosome pairing, meiotic crossover formation, and accurate chromosome segregation, thereby ensuring normal gametogenesis and fertility. Deficiency or mutation of SYCE2 disrupts synaptonemal complex formation, resulting in defective meiosis, germ cell apoptosis, and infertility.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SYCE2-Specific antibody 16859-1-AP | Download protocol |
| IHC protocol for SYCE2-Specific antibody 16859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
