验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14565-1-AP targets SVOP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag6134 Product name: Recombinant human SVOP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 261-548 aa of BC033587 Sequence: SARYDVLSGNQEKAIATLKRIATENGAPMPLGKLIISRQEDRGKMRDLFTPHFRWTTLLLWFIWFSNAFSYYGLVLLTTELFQAGDVCGISSRKKAVEAKCSLACEYLSEEDYMDLLWTTLSEFPGVLVTLWIIDRLGRKKTMALCFVIFSFCSLLLFICVGRNVLTLLLFIARAFISGGFQAAYVYTPEVYPTATRALGLGTCSGMARVGALITPFIAQVMLESSVYLTLAVYSGCCLLAALASCFLPIETKGRGLQESSHREWGQEMVGRGMHGAGVTRSNSGSQE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SV2 related protein homolog (rat) |
| 别名 | SLC22B4, SV2-related protein, Synaptic vesicle 2-related protein |
| 计算分子量 | 61 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC033587 |
| 基因名称 | SVOP |
| Gene ID (NCBI) | 55530 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N4V2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SVOP (Synaptic Vesicle 2-Related Protein) is an evolutionarily conserved, nonglycosylated transmembrane protein localized to synaptic vesicles and microvesicles in neurons and endocrine cells. Distantly related to SV2, it contains 12 transmembrane domains and shares homology with organic ion transporters. SVOP binds adenine nucleotides, particularly NAD, and is thought to contribute to synaptic vesicle function, though its precise physiological role remains to be fully elucidated. (PMID: 9801366)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SVOP antibody 14565-1-AP | Download protocol |
| WB protocol for SVOP antibody 14565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
