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SVCT2 Polyclonal antibody

SVCT2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27019-1-AP

Print datasheet

Synonyms

ascorbate transporter, hSVCT2, KIAA0238, NBTL1, SLC23A2, SVCT2, YSPL2

验证数据展示

  • WB analysis using 27019-1-AP

    WB analysis using 27019-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27019-1-AP (SVCT2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 27019-1-AP

    WB analysis using 27019-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27019-1-AP (SVCT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 27019-1-AP

    IHC staining of human colon cancer using 27019-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27019-1-AP

    IHC staining of mouse brain using 27019-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27019-1-AP

    IHC staining of mouse brain using 27019-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 27019-1-AP

    IHC staining of human colon cancer using 27019-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 27019-1-AP

    IHC staining of human colon cancer using 27019-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCaco-2 cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27019-1-AP targets SVCT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SVCT2 fusion protein Ag25504 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 23 (nucleobase transporters), member 2
Synonyms ascorbate transporter, hSVCT2, KIAA0238, NBTL1, SLC23A2, SVCT2, YSPL2
Calculated Molecular Weight 70 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC013112
Gene Symbol SLC23A2
Gene ID (NCBI) 9962
RRIDAB_2918116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGH3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SVCT2 antibody 27019-1-APDownload protocol
IHC protocol for SVCT2 antibody 27019-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols