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验证数据展示

  • WB analysis using 27019-1-AP

    WB analysis using 27019-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27019-1-AP (SVCT2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 27019-1-AP

    WB analysis using 27019-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27019-1-AP (SVCT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 27019-1-AP

    IHC staining of human colon cancer using 27019-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27019-1-AP

    IHC staining of mouse brain using 27019-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27019-1-AP

    IHC staining of mouse brain using 27019-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 27019-1-AP

    IHC staining of human colon cancer using 27019-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 27019-1-AP

    IHC staining of human colon cancer using 27019-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SVCT2 Polyclonal antibody

SVCT2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27019-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

ascorbate transporter, hSVCT2, KIAA0238, NBTL1, SLC23A2, SVCT2, YSPL2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27019-1-AP targets SVCT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SVCT2 fusion protein Ag25504 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 23 (nucleobase transporters), member 2
别名 ascorbate transporter, hSVCT2, KIAA0238, NBTL1, SLC23A2, SVCT2, YSPL2
计算分子量 70 kDa
观测分子量70 kDa
GenBank蛋白编号BC013112
基因名称 SLC23A2
Gene ID (NCBI) 9962
RRIDAB_2918116
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGH3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SVCT2 antibody 27019-1-APDownload protocol
IHC protocol for SVCT2 antibody 27019-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

J Periodontal Res

Role of sodium-dependent vitamin C transporter 2 in human periodontal ligament fibroblasts

Authors - Tomoko Kanda
View Article
mouseWB

Adv Sci (Weinh)

Vitamin C-Dependent Intergenerational Inheritance of Enhanced Endurance Performance Following Maternal Exercise

Authors - Haiwang Shi
View Article