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SV2C Polyclonal antibody

SV2C Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 21271-1-AP

Print datasheet

Synonyms

KIAA1054, SV2C

验证数据展示

  • IHC staining of mouse cerebellum using 21271-1-AP

    IHC staining of mouse cerebellum using 21271-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21271-1-AP (SV2C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 21271-1-AP

    IHC staining of mouse cerebellum using 21271-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21271-1-AP (SV2C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse brain using 21271-1-AP

    WB analysis of mouse brain using 21271-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21271-1-AP (SV2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 21271-1-AP

    IP experiment of mouse brain using 21271-1-AP

    IP result of anti-SV2C (IP:21271-1-AP, 4ug; Detection:21271-1-AP 1:500) with mouse brain tissue lysate 3440ug.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse skin tissue, C6 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21271-1-AP is SV2C Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen SV2C fusion protein Ag15745 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptic vesicle glycoprotein 2C
Synonyms KIAA1054, SV2C
Calculated Molecular Weight 727 aa, 82 kDa
Observed Molecular Weight 80-95 kDa
GenBank Accession NumberBC100826
Gene Symbol SV2C
Gene ID (NCBI) 22987
RRIDAB_10755297
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ496J9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SV2C antibody 21271-1-APDownload protocol
IHC protocol for SV2C antibody 21271-1-APDownload protocol
IP protocol for SV2C antibody 21271-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols