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验证数据展示

  • WB analysis of human brain using 14624-1-AP

    WB analysis of human brain using 14624-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14624-1-AP (SV2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 14624-1-AP

    IHC staining of human brain using 14624-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14624-1-AP (SV2B antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 14624-1-AP

    IHC staining of human brain using 14624-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14624-1-AP (SV2B antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse brain using 14624-1-AP

    IP experiment of mouse brain using 14624-1-AP

    IP result of anti-SV2B (IP:14624-1-AP, 4ug; Detection:14624-1-AP 1:500) with mouse brain tissue lysate 4000ug.

SV2B Polyclonal antibody

SV2B Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14624-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

别名

HsT19680, KIAA0735, SV2B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14624-1-AP targets SV2B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SV2B fusion protein Ag6137 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptic vesicle glycoprotein 2B
别名 HsT19680, KIAA0735, SV2B
计算分子量 77 kDa
观测分子量 77 kDa
GenBank蛋白编号BC030011
基因名称 SV2B
Gene ID (NCBI) 9899
RRIDAB_10641040
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L1I2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SV2B antibody 14624-1-APDownload protocol
IHC protocol for SV2B antibody 14624-1-APDownload protocol
IP protocol for SV2B antibody 14624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Neurol

Environmental Enrichment Upregulates Striatal Synaptic Vesicle-Associated Proteins and Improves Motor Function.

Authors - Suk-Young Song
View Article
mouseWB

Neurosci Bull

Mutant Huntingtin Causes a Selective Decrease in the Expression of Synaptic Vesicle Protein 2C.

Authors - Chaohua Peng
View Article
humanIHC,IF

Sci Rep

SV2B/miR-34a/miR-128 axis as prognostic biomarker in glioblastoma multiforme

Authors - D Mustafov
View Article