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SV2B Polyclonal antibody

SV2B Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 14624-1-AP

Print datasheet

Synonyms

HsT19680, KIAA0735, SV2B

验证数据展示

  • WB analysis of human brain using 14624-1-AP

    WB analysis of human brain using 14624-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14624-1-AP (SV2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 14624-1-AP

    IHC staining of human brain using 14624-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14624-1-AP (SV2B antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 14624-1-AP

    IHC staining of human brain using 14624-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14624-1-AP (SV2B antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse brain using 14624-1-AP

    IP experiment of mouse brain using 14624-1-AP

    IP result of anti-SV2B (IP:14624-1-AP, 4ug; Detection:14624-1-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14624-1-AP targets SV2B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen SV2B fusion protein Ag6137 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptic vesicle glycoprotein 2B
Synonyms HsT19680, KIAA0735, SV2B
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC030011
Gene Symbol SV2B
Gene ID (NCBI) 9899
RRIDAB_10641040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L1I2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SV2B antibody 14624-1-APDownload protocol
IHC protocol for SV2B antibody 14624-1-APDownload protocol
IP protocol for SV2B antibody 14624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Neurol

Environmental Enrichment Upregulates Striatal Synaptic Vesicle-Associated Proteins and Improves Motor Function.

Authors - Suk-Young Song
View Article
mouseWB

Neurosci Bull

Mutant Huntingtin Causes a Selective Decrease in the Expression of Synaptic Vesicle Protein 2C.

Authors - Chaohua Peng
View Article