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验证数据展示

  • WB analysis using 83052-6-RR

    WB analysis using 83052-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83052-6-RR

    WB analysis using 83052-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 83052-6-RR

    WB analysis of MCF-7 using 83052-6-RR

    WB result of SURF1 antibody (83052-6-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SURF1 transfected MCF-7 cells.

  • FC experiment of HepG2 using 83052-6-RR

    FC experiment of HepG2 using 83052-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-6-RR, Clone:230210E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83052-6-RR

    Affinity and Kinetic Characterization of 83052-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83052-6-RR against Human SURF1 were performed. The affinity constant is 0.509 nM.

  • Featured Product
  • KD/KO验证
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SURF1 Recombinant monoclonal antibody

SURF1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83052-6-RR

产品说明书

CloneNo. 230210E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue, rat heart tissue
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83052-6-RR targets SURF1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3959

Product name: Recombinant human SURF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC028314

Sequence: MAAVAALQLGLRAAGLGRAPASAAWRSVLRVSPRPGVAWRPSRCGSSAAEASATKAEDDSFLQWVLLLIPVTAFGLGTWQVQRRKWKLNLIAELESRVLAEPVPLPADPMELKNLEYRPVKVRGCFDHSKELYMMPRTMVDPVREAREGGLISSSTQSGAYVVTPFHCTDLGVTILVNRGFVPRKKVNPETRQKGQIEGEVDLIGMVRLTETRQPFVPENNPERNHWHYRDLEAMARITGAEPIFIDANFQSTVPGGPIGGQTRVTLRNEHLQYIVTWYGLSAATSYLWFKKFLRGTPGV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 surfeit 1
别名 Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210E12
计算分子量 33 kDa
观测分子量30-31 kDa
GenBank蛋白编号BC028314
基因名称 SURF1
Gene ID (NCBI) 6834
RRIDAB_3670776
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15526
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SURF1 antibody 83052-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols