• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SURF1 Recombinant antibody

SURF1 Recombinant Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230210F11

Cat No : 83052-5-RR

Print datasheet

Synonyms

SURF 1, SURF1, surfeit 1, Surfeit locus protein 1

验证数据展示

  • IHC staining of human liver cancer using 83052-5-RR

    IHC staining of human liver cancer using 83052-5-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 83052-5-RR

    IHC staining of human liver cancer using 83052-5-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 83052-5-RR

    IHC staining of mouse heart using 83052-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 83052-5-RR

    IHC staining of mouse heart using 83052-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 83052-5-RR

    IF Staining of HeLa using 83052-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of HeLa using 83052-5-RR

    IF Staining of HeLa using 83052-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive IHC detected inhuman liver cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83052-5-RR targets SURF1 in IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, IHC, ELISA
Tested Reactivity Human, mouse
Immunogen SURF1 fusion protein Ag3959 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name surfeit 1
Synonyms SURF 1, SURF1, surfeit 1, Surfeit locus protein 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight30-31 kDa
GenBank Accession NumberBC028314
Gene Symbol SURF1
Gene ID (NCBI) 6834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15526
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SURF1 antibody 83052-5-RRDownload protocol
IF protocol for SURF1 antibody 83052-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols