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  • Featured Product
  • KD/KO Validated

SUPT5H Polyclonal antibody

SUPT5H Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16511-1-AP

Print datasheet

Synonyms

DSIF large subunit, DSIF p160, hSPT5, SPT5, SPT5H, SUPT5H, Tat cotransactivator 1 protein, Tat CT1 protein

验证数据展示

  • WB analysis of HeLa using 16511-1-AP

    WB analysis of HeLa using 16511-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16511-1-AP (SUPT5H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16511-1-AP

    IP experiment of HeLa using 16511-1-AP

    IP result of anti-SUPT5H (IP:16511-1-AP, 4ug; Detection:16511-1-AP 1:500) with HeLa cells lysate 3200ug.

  • IF Staining of HepG2 using 16511-1-AP

    IF Staining of HepG2 using 16511-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16511-1-AP (SUPT5H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 16511-1-AP

    IF Staining of HepG2 using 16511-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUPT5H antibody (16511-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16511-1-AP targets SUPT5H in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SUPT5H fusion protein Ag9728 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name suppressor of Ty 5 homolog (S. cerevisiae)
Synonyms DSIF large subunit, DSIF p160, hSPT5, SPT5, SPT5H, SUPT5H, Tat cotransactivator 1 protein, Tat CT1 protein
Calculated Molecular Weight 1087 aa, 121 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC024203
Gene Symbol SUPT5H
Gene ID (NCBI) 6829
RRIDAB_2878268
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00267
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SUPT5H, also named as DSIF p160, is a 1087 amino acid protein, which belongs to the SPT5 family. SUPT5H localizes in the nucleus and is ubiquitously expressed. SUPT5H is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H is modified through phosphorylation and methylated, so the molecular weight of SUPT5H might be 140-150 kDa.

实验方案

Product Specific Protocols
WB protocol for SUPT5H antibody 16511-1-APDownload protocol
IF protocol for SUPT5H antibody 16511-1-APDownload protocol
IP protocol for SUPT5H antibody 16511-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.

Authors - Shibin Hu
  • KD Validated
View Article
humanWB

Mol Cell

INTAC endonuclease and phosphatase modules differentially regulate transcription by RNA polymerase II

Authors - Shibin Hu
View Article