验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| CoIP | See 1 publications below |
产品信息
26434-1-AP targets SUNC1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag23780 Product name: Recombinant human SUNC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-228 aa of BC026189 Sequence: MSGKTKARRAAMFFRRCSEDASGSASGNALLSEDENPDANGVTRSWKIILSTMLTLTFLLVGLLNHQWLKETDVPQKSRQLYAIIAEYGSRLYKYQARLRMPKEQLELLKKESQNLENNFRQILFLVEQIDVLKALLRDMKDGMDNNHNWNTHGDPVEDPDHTEEVSNLVNYVLKKLREDQVEMADYALKSAGASIIEAGTSESYKNNKAKLYWHGIGFLNHEMPPDI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Sad1 and UNC84 domain containing 1 |
| 别名 | SUN3, SUN domain-containing protein 3, Sad1/unc-84 domain-containing protein 1 |
| 观测分子量 | 37-41 kDa |
| GenBank蛋白编号 | BC026189 |
| 基因名称 | SUNC1 |
| Gene ID (NCBI) | 256979 |
| RRID | AB_2880513 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TAQ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SUNC1 encodes SUN3 which is a member of SUN domain protein family, an evolutionary conserved family of inner nuclear membrane (INM) proteins that share a common C-terminal motif. SUN3 is mainly expressed in testis and is involved in sperm head formation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SUNC1 antibody 26434-1-AP | Download protocol |
| IP protocol for SUNC1 antibody 26434-1-AP | Download protocol |
| WB protocol for SUNC1 antibody 26434-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. | ||
Cell Mol Life Sci CCDC28A deficiency causes sperm head defects, reduced sperm motility and male infertility in mice | ||
J Biol Chem The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis. |
