Validation Data Gallery
Product Information
The ChromoTek SUMO1-Trap Magnetic Agarose consists of an anti-SUMO1 NANOBODY®/VHH, which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of SUMO1 proteins from cell extracts of various organisms.
| Description | Immunoprecipitation of SUMO1 proteins with anti-SUMO1 Nanobody conjugated to magnetic agarose beads. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
| Applications | IP, Co-IP |
| Specificity/Target | Binds specifically to human SUMO1 (Small Ubiquitin-like Modifier 1). The ChromoTek SUMO1-Trap Agarose will immunoprecipitate both monovalent SUMO1 and SUMO1 chains formed by the process of SUMOylation. In addition to human SUMO1, it will also bind SUMO1 homologs from other mammals. |
| Binding Capacity | 20 μg of SUMO1 protein (~40 kDa) per 25 μL bead slurry |
| Conjugate | Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads) |
| Elution buffer | 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5 |
| Wash Buffer Compatibility | / |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | / |
| Compatibility with mass spectrometry | The SUMO1-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_3716416 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze! |
Documentation
| SDS |
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| s1tma-SDS-SUMO1-Trap Magnetic Agarose (EN) |
| Datasheet |
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| SUMO1-Trap Magnetic Agarose Datasheet |