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验证数据展示

  • WB analysis using 29185-1-AP (same clone as 29185-1-PBS)

    WB analysis using 29185-1-AP (same clone as 29185-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29185-1-AP (SULT2B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29185-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 29185-1-AP (same clone as 29185-1-PBS)

    IF Staining of MCF-7 using 29185-1-AP (same clone as 29185-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SULT2B1 antibody (29185-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29185-1-PBS in a different storage buffer formulation.

SULT2B1 Polyclonal antibody, PBS Only

SULT2B1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 29185-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29185-1-PBS targets SULT2B1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30209

Product name: Recombinant human SULT2B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 283-365 aa of BC034694

Sequence: NHFTVAQSEAFDRAYRKQMRGMPTFPWDEDPEEDGSPDPEPSPEPEPKPSLEPNTSLEREPRPNSSPSPSPGQASETPHPRPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sulfotransferase family, cytosolic, 2B, member 1
别名
计算分子量 365 aa, 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC034694
基因名称 SULT2B1
Gene ID (NCBI) 6820
RRIDAB_2918245
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00204
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate.

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