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验证数据展示

  • IHC staining of human breast cancer using 12522-1-AP (same clone as 12522-1-PBS)

    IHC staining of human breast cancer using 12522-1-AP (same clone as 12522-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12522-1-AP (same clone as 12522-1-PBS)

    IHC staining of human breast cancer using 12522-1-AP (same clone as 12522-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • WB analysis of human lung using 12522-1-AP (same clone as 12522-1-PBS)

    WB analysis of human lung using 12522-1-AP (same clone as 12522-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 12522-1-AP (same clone as 12522-1-PBS)

    IP experiment of mouse liver using 12522-1-AP (same clone as 12522-1-PBS)

    IP result of anti-SULT1E1 (IP:12522-1-AP, 4ug; Detection:12522-1-AP 1:400) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SULT1E1 Polyclonal antibody, PBS Only

SULT1E1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12522-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12522-1-PBS targets SULT1E1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3216

Product name: Recombinant human SULT1E1 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-294 aa of BC027956

Sequence: MNSELDYYEKFEEVHGILMYKDFVKYWDNVEAFQARPDDLVIATYPKSGTTWVSEIVYMIYKEGDVEKCKEDVIFNRIPFLECRKENLMNGVKQLDEMNSPRIVKTHLPPELLPASFWEKDCKIIYLCRNAKDVAVSFYYFFLMVAGHPNPGSLPEFVEKFMQGQVPYGSWYKHVKSWWEKGKSPRVLFLFYEDLKEDIRKEVIKLIHFLERKPSEELVDRIIHHTSFQEMKNNPSTNYTTLPDEIMNQKLSPFMRKGITGDWKNHFTVALNEKFDKHYEQQMKESTLKFRTEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sulfotransferase family 1E, estrogen-preferring, member 1
别名
计算分子量 294 aa, 35 kDa
观测分子量 32-35 kDa
GenBank蛋白编号BC027956
基因名称 SULT1E1
Gene ID (NCBI) 6783
RRIDAB_2302772
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49888
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SULT1E1, also known as estrogen sulfotransferase, catalyzes the sulfation of endogenous estrogens as well as xenobiotic estrogen-like chemicals, converting them into the inactive form. SULT1E1 is a 33-35 kDa cytosolic protein that has been detected in human hepatic and nonhepatic tissues (17035602). SULT1E1 is expressed in breast and endometrial tissues, and some studies have investigated its implication in tumor development. Higher expression of SULT1E1 was observed in breast cancer tissues compared with normal breast tissues (22380844). This antibody detected a major band around 35 kD in lysates of mouse kidney, human liver and so on.

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