验证数据展示
经过测试的应用
| Positive WB detected in | human colon tissue, BxPC-3 cells, HepG2 cells, human pancreas tissue, mouse pancreas tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11376-1-AP targets SULT1A4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1935 Product name: Recombinant human SULT1A4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-295 aa of BC014471 Sequence: MELIQDTSRPPLEYVKGVPLIKYFAEALGPLQSFQARPDDLLINTYPKSGTTWVSQILDMIYQGGDLEKCNRAPIYVRVPFLEVNDPGEPSGLETLKDTPPPRLIKSHLPLALLPQTLLDQKVKVVYVARNPKDVAVSYYHFHRMEKAHPEPGTWDSFLEKFMAGEVSYGSWYQHVQEWWELSRTHPVLYLFYEDMKENPKREIQKILEFVGRSLPEETMDFMVQHTSFKEMKKNPMTNYTTVPQELMDHSISPFMRKGMAGDWKTTFTVAQNERFDADYAEKMAGCSLSFRSEL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sulfotransferase family, cytosolic, 1A, phenol-preferring, member 4 |
| 别名 | Monoamine-sulfating phenol sulfotransferase, M PST, HAST3, EC:2.8.2.1, Catecholamine-sulfating phenol sulfotransferase |
| 计算分子量 | 295 aa, 34 kDa |
| 观测分子量 | 30-34 kDa |
| GenBank蛋白编号 | BC014471 |
| 基因名称 | SULT1A4 |
| Gene ID (NCBI) | 445329 |
| RRID | AB_2197610 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P0DMN0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SULT1A4 antibody 11376-1-AP | Download protocol |
| IHC protocol for SULT1A4 antibody 11376-1-AP | Download protocol |
| WB protocol for SULT1A4 antibody 11376-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
