验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells |
| Positive IF/ICC detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy SUGT1 Ready-To-Use IHC Kit. SUGT1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84963-5-RR targets SUGT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag2275 Product name: Recombinant human SUGT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-333 aa of BC000911 Sequence: MAAAAAGTATSQRFFQSFSDALIDEDPQAALEELTKALEQKPDDAQYYCQRAYCHILLGNYCVAVADAKKSLELNPNNSTAMLRKGICEYHEKNYAAALETFTEGQKLDSADANFSVWIKRCQEAQNGSESEVWTHQSKIKYDWYQTESQVVITLMIKNVQKNDVNVEFSEKELSALVKLPSGEDYNLKLELLHPIIPEQSTFKVLSTKIEIKLKKPEAVRWEKLEGQGDVPTPKQFVADVKNLYPSSSPYTRNWDKLVGEIKEEEKNEKLEGDAALNRLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKKY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) |
| 别名 | 242590G3, Protein 40 6 3, Protein 40-6-3, Protein SGT1 homolog, Sgt1 |
| 计算分子量 | 41 kDa |
| 观测分子量 | 38-41 kDa |
| GenBank蛋白编号 | BC000911 |
| 基因名称 | SUGT1 |
| Gene ID (NCBI) | 10910 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9Y2Z0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SUGT1 antibody 84963-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
