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STXBP5 Polyclonal antibody

STXBP5 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 24512-1-AP

Print datasheet

Synonyms

LGL3, LLGL3, Nbla04300, STXBP5, Syntaxin binding protein 5, Tomosyn 1

验证数据展示

  • WB analysis of rat brain using 24512-1-AP

    WB analysis of rat brain using 24512-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 24512-1-AP (STXBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of rat brain using 24512-1-AP

    IP experiment of rat brain using 24512-1-AP

    IP result of anti-STXBP5 (IP:24512-1-AP, 5ug; Detection:24512-1-AP 1:300) with rat brain tissue lysate 4000ug.

  • IF Staining of SH-SY5Y using 24512-1-AP

    IF Staining of SH-SY5Y using 24512-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 24512-1-AP (STXBP5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inrat brain tissue
Positive IP detected inrat brain tissue
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24512-1-AP is STXBP5 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IP, WB,ELISA
Tested Reactivity human, rat
Immunogen STXBP5 fusion protein Ag21629 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name syntaxin binding protein 5 (tomosyn)
Synonyms LGL3, LLGL3, Nbla04300, STXBP5, Syntaxin binding protein 5, Tomosyn 1
Calculated Molecular Weight 1151 aa, 128 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC113382
Gene Symbol STXBP5
Gene ID (NCBI) 134957
RRIDAB_2879582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T5C0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin-binding protein 5 (STXBP5, also known as tomosyn) is a cytosolic syntaxin-binding protein that can displace MUNC18 from syntaxin-1. It is a soluble R-SNARE protein that sequesters target SNAREs through the C-terminal VAMP-like domain. STXBP5 plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release.

实验方案

Product Specific Protocols
WB protocol for STXBP5 antibody 24512-1-APDownload protocol
IF protocol for STXBP5 antibody 24512-1-APDownload protocol
IP protocol for STXBP5 antibody 24512-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols