• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 14231-1-AP

    WB analysis using 14231-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14231-1-AP (STXBP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14231-1-AP

    IHC staining of human pancreas cancer using 14231-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14231-1-AP (STXBP4 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with STXBP4 antibody included
  • IHC staining of human pancreas cancer using 14231-1-AP

    IHC staining of human pancreas cancer using 14231-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14231-1-AP (STXBP4 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with STXBP4 antibody included
  • WB analysis using 14231-1-AP

    WB analysis using 14231-1-AP

    PC-3 lysates were subjected to SDS PAGE followed by western blot with 14231-1-AP (STXBP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 14231-1-AP

    IF Staining of BxPC-3 using 14231-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using STXBP4 antibody (14231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

STXBP4 Polyclonal antibody

STXBP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14231-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

STX4-interacting protein, Synip, Syntaxin 4-interacting protein, Syntaxin-binding protein 4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, PC-3 cells, HuH-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy STXBP4 Ready-To-Use IHC Kit. STXBP4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14231-1-AP targets STXBP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5471

Product name: Recombinant human STXBP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-245 aa of BC041485

Sequence: MIGVSFEEAKSIITGAKLSTRLESAWEIAFIRQKSDNIQPENLSCTSLIEASGEYGPQASTLSLFSSPPEILIPKTSSTPKTNNDILSSCEIKTGYNKTVQIPITSENSTVGLSNTDVASAWTENYGLQEKISLNPSVRFKAEKLEMALNYLGIQPTKEQHQALRQQVQADSKGTVSFGDFVQVARNLFCLQLDEVNVGAHEISNILDSQLLPCDSSEADEMERLKCERDDALKEVNTLKWPGQN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 syntaxin binding protein 4
别名 STX4-interacting protein, Synip, Syntaxin 4-interacting protein, Syntaxin-binding protein 4
计算分子量 62 kDa
观测分子量 62 kDa
GenBank蛋白编号BC041485
基因名称 STXBP4
Gene ID (NCBI) 252983
RRIDAB_2196980
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZWJ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STXBP4, also known as Synip, plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. STXBP4 inhibits the translocation of GLUT4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2(PMID: 10394363).

实验方案

Product Specific Protocols
IF protocol for STXBP4 antibody 14231-1-APDownload protocol
IHC protocol for STXBP4 antibody 14231-1-APDownload protocol
WB protocol for STXBP4 antibody 14231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...