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验证数据展示

  • WB analysis using 14231-1-AP

    WB analysis using 14231-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14231-1-AP (STXBP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14231-1-AP

    IHC staining of human pancreas cancer using 14231-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14231-1-AP (STXBP4 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with STXBP4 antibody included
  • IHC staining of human pancreas cancer using 14231-1-AP

    IHC staining of human pancreas cancer using 14231-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14231-1-AP (STXBP4 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with STXBP4 antibody included
  • WB analysis using 14231-1-AP

    WB analysis using 14231-1-AP

    PC-3 lysates were subjected to SDS PAGE followed by western blot with 14231-1-AP (STXBP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 14231-1-AP

    IF Staining of BxPC-3 using 14231-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using STXBP4 antibody (14231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

STXBP4 Polyclonal antibody

STXBP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14231-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

STX4-interacting protein, Synip, Syntaxin 4-interacting protein, Syntaxin-binding protein 4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, PC-3 cells, HuH-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy STXBP4 Ready-To-Use IHC Kit. STXBP4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14231-1-AP targets STXBP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5471

Product name: Recombinant human STXBP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-245 aa of BC041485

Sequence: MIGVSFEEAKSIITGAKLSTRLESAWEIAFIRQKSDNIQPENLSCTSLIEASGEYGPQASTLSLFSSPPEILIPKTSSTPKTNNDILSSCEIKTGYNKTVQIPITSENSTVGLSNTDVASAWTENYGLQEKISLNPSVRFKAEKLEMALNYLGIQPTKEQHQALRQQVQADSKGTVSFGDFVQVARNLFCLQLDEVNVGAHEISNILDSQLLPCDSSEADEMERLKCERDDALKEVNTLKWPGQN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 syntaxin binding protein 4
别名 STX4-interacting protein, Synip, Syntaxin 4-interacting protein, Syntaxin-binding protein 4
计算分子量 62 kDa
观测分子量 62 kDa
GenBank蛋白编号BC041485
基因名称 STXBP4
Gene ID (NCBI) 252983
RRIDAB_2196980
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZWJ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for STXBP4 antibody 14231-1-APDownload protocol
IHC protocol for STXBP4 antibody 14231-1-APDownload protocol
WB protocol for STXBP4 antibody 14231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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