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STXBP3 Polyclonal antibody

STXBP3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 13764-1-AP

Print datasheet

Synonyms

MUNC18 3, MUNC18C, Platelet Sec1 protein, Protein unc 18 homolog 3, Protein unc 18 homolog C, PSP, STXBP3, syntaxin binding protein 3, UNC 18C, Unc18 3

验证数据展示

  • WB analysis of HepG2 using 13764-1-AP

    WB analysis of HepG2 using 13764-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13764-1-AP (STXBP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 13764-1-AP

    IHC staining of human lymphoma using 13764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13764-1-AP (STXBP3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lymphoma using 13764-1-AP

    IHC staining of human lymphoma using 13764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13764-1-AP (STXBP3 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of K-562 using 13764-1-AP

    IP experiment of K-562 using 13764-1-AP

    IP result of anti-STXBP3 (IP:13764-1-AP, 4ug; Detection:13764-1-AP 1:500) with K-562 cells lysate 1200ug.

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

13764-1-AP targets STXBP3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen STXBP3 fusion protein Ag4747 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name syntaxin binding protein 3
Synonyms MUNC18 3, MUNC18C, Platelet Sec1 protein, Protein unc 18 homolog 3, Protein unc 18 homolog C, PSP, STXBP3, syntaxin binding protein 3, UNC 18C, Unc18 3
Calculated Molecular Weight 592 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC038099
Gene Symbol STXBP3
Gene ID (NCBI) 6814
RRIDAB_2196832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00186
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STXBP3 antibody 13764-1-APDownload protocol
IHC protocol for STXBP3 antibody 13764-1-APDownload protocol
IP protocol for STXBP3 antibody 13764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Diabetes

PMID: 20460426

Authors - Pontus Boström
View Article
mouseWB

J Biol Chem

microRNA-15b contributes to depression-like behavior in mice by affecting synaptic protein levels and function in the nucleus accumbens.

Authors - Li Guo
View Article
ratWB

Neuroscience

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Xing-Lian Duan
View Article