验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, A375 cells, human brain tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue, mouse pancreas tissue |
| Positive IP detected in | A375 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
12206-1-AP targets Syntaxin 8 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 |
CatNo: Ag2850 Product name: Recombinant human STX8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-211 aa of BC009713 Sequence: MAPDPWFSTYDSTCQIAQEIAEKIQQRNQYERKGEKAPKLTVTIRALLQNLKEKIALLKDLLLRAVSTHQITQLEGDRRQNLLDDLVTRERLLLASFKNEGAEPDLIRSSLMSEEAKRGAPNPWLFEEPEETRGLGFDEIRQQQQKIIQEQDAGLDALSSIISRQKQMGQEIGNELDEQNEIIDDLANLVENTDEKLRNETRRVNMVDRKS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syntaxin 8 |
| 别名 | Syntaxin-8, STX8 |
| 计算分子量 | 236 aa, 27 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC009713 |
| 基因名称 | Syntaxin 8 |
| Gene ID (NCBI) | 9482 |
| RRID | AB_2196671 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UNK0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syntaxin 8 (STX8), also known as CIP-1-associated regulator of cyclin B (CARB ), is a protein belonging to the syntaxin family. The gene of STX8 maps to chromosomal band 17p12 and it encodes a 236-amino-acid protein containing 1 t-SNARE coiled-coil homology domain. STX8 mRNA is broadly expressed in multiple human tissues, including the heart, brain, kidney, liver, lung, placenta, skeletal muscle, spleen, and pancreas (PMID: 10198254). STX8 is involved in protein trafficking from early to late endosomes via vesicle fusion and exocytosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Syntaxin 8 antibody 12206-1-AP | Download protocol |
| IP protocol for Syntaxin 8 antibody 12206-1-AP | Download protocol |
| WB protocol for Syntaxin 8 antibody 12206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol VAMP4 regulates insulin levels by targeting secretory granules to lysosomes
| ||
PLoS One The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification. |
