验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue, MDCK cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human medulloblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12322-1-AP targets Syntaxin 7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, canine |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2975 Product name: Recombinant human STX7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC011975 Sequence: MSYTPGVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQVQVQDEEITEDDLRLIHERESSIRQLEADIMDINEIFKDLGMMIHEQGDVIDSIEANVENAEVHVQQANQQLSRAADYQRKSRKTLCIIILI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syntaxin 7 |
| 别名 | Syntaxin-7, STX7 |
| 计算分子量 | 261 aa, 30 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC011975 |
| 基因名称 | Syntaxin 7 |
| Gene ID (NCBI) | 8417 |
| RRID | AB_2239979 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15400 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syntaxin 7() is a SNARE protein localized to late endosomes and lysosomes, mediating vesicle fusion and intracellular trafficking. It plays essential roles in autophagy, pathogen degradation, and cholesterol transport through interactions with VAMP8 and Vti1b.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| IHC protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| IP protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| WB protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation. | ||
J Cell Biol VAMP4 regulates insulin levels by targeting secretory granules to lysosomes
| ||
Biochem J Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. | ||
Cell Logist Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins. | ||
J Biol Chem Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome |
