验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, rat kidney tissue, HEK-293 cells, Jurkat cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
产品信息
14988-1-AP targets Syntaxin 4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag6973 Product name: Recombinant human STX4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC002436 Sequence: MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syntaxin 4 |
| 别名 | Syntaxin-4, STX4A, STX4, Renal carcinoma antigen NY-REN-31 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 35-37 kDa |
| GenBank蛋白编号 | BC002436 |
| 基因名称 | STX4 |
| Gene ID (NCBI) | 6810 |
| RRID | AB_2286910 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12846 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Syntaxin 4 antibody 14988-1-AP | Download protocol |
| IP protocol for Syntaxin 4 antibody 14988-1-AP | Download protocol |
| WB protocol for Syntaxin 4 antibody 14988-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Reduced oligodendrocyte exosome secretion in multiple system atrophy involves SNARE dysfunction. | ||
Front Immunol Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing. | ||
Int J Biol Macromol PKCε controls the fusion of secretory vesicles in mast cells in a phosphatidic acid-dependent mode. | ||
Br J Pharmacol CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells. | ||
J Biol Chem Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin. | ||
J Transl Med Angiopoietin2-mediated caveolin1 phosphorylation regulating transcytosis of renal tubular epithelial cell contributes to the occurrence of albuminuria under high glucose exposure. |
