验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55033-1-AP targets Syntaxin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syntaxin 2 |
| 别名 | STX2, EPIM, Epimorphin, EPM, STX2A |
| 计算分子量 | 33 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | NM_001980 |
| 基因名称 | Syntaxin 2 |
| Gene ID (NCBI) | 2054 |
| RRID | AB_11182166 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P32856 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syntaxin 2 (STX2), also named as EPIM, STX2A, STX2B and STX2C, belongs to the syntaxin family. It is essential for epithelial morphogenesis. STX2 is a member of the SNARE membrane fusion machinery which localized to the midbody during cytokinesis in rat and canine kidney cell lines. Inhibition of STX2 and Vamp8 function by overexpression of nonmembrane-anchored mutants caused failure of cytokinesis leading to the formation of binucleated cells. Time-lapse microscopy showed that only midbody abscission and not further upstream events, such as furrowing, were affected. The antibody is specific to STX2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Syntaxin 2 antibody 55033-1-AP | Download protocol |
| WB protocol for Syntaxin 2 antibody 55033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis STX2 promotes colorectal cancer metastasis through a positive feedback loop that activates the NF-κB pathway.
| ||
J Cell Sci Syntaxin 7 contributes to breast cancer cell invasion by promoting invadopodia formation. | ||
Life Sci STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.
| ||
J Cancer Syntaxin 2 promotes colorectal cancer growth by increasing the secretion of exosomes. | ||
Neuroscience SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain. |
