验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, DU 145 cells, human placenta tissue, human skeletal muscle tissue, Jurkat cells, L02 cells, mouse placenta tissue, rat lung tissue |
| Positive IP detected in | DU 145 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13301-1-AP targets Syntaxin 11 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4097 Product name: Recombinant human STX11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-287 aa of BC033519 Sequence: MKDRLAELLDLSKQYDQQFPDGDDEFDSPHEDIVFETDHILESLYRDIRDIQDENQLLVADVKRLGKQNARFLTSMRRLSSIKRDTNSIAKAIKARGEVIHCKLRAMKELSEAAEAQHGPHSAVARISRAQYNALTLTFQRAMHDYNQAEMKQRDNCKIRIQRQLEIMGKEVSGDQIEDMFEQGKWDVFSENLLADVKGARAALNEIESRHRELLRLESRIRDVHELFLQMAVLVEKQADTLNVIELNVQKTVDYTGQAKAQVRKAVQYEEKNPCRTLCCFCCPCLK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syntaxin 11 |
| 别名 | Syntaxin-11, STX11 |
| 计算分子量 | 287 aa, 33 kDa |
| 观测分子量 | 33-35 kDa |
| GenBank蛋白编号 | BC033519 |
| 基因名称 | Syntaxin 11 |
| Gene ID (NCBI) | 8676 |
| RRID | AB_2198214 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75558 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syntaxin 11 (STX11) belongs to the syntaxin family. It interacts with the SNARE proteins SNAP-23 and VAMP. STX11 behaves as an integral membrane protein despite the lack of a traditional transmembrane domain. The detection of STX11 in human cytotoxic lymphocytes suggests that this protein could play a role in the process leading to degranulation of secretory lysosomes (PMID:17525286).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Syntaxin 11 antibody 13301-1-AP | Download protocol |
| IP protocol for Syntaxin 11 antibody 13301-1-AP | Download protocol |
| WB protocol for Syntaxin 11 antibody 13301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion
| ||
J Exp Med Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency. | ||
Proc Natl Acad Sci U S A Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation. | ||
J Cell Sci Chaperoning of closed syntaxin-3 through Lys46 and Glu59 in domain-1 of Munc18 is indispensable for mast cell exocytosis. | ||
Front Immunol Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing. |
