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验证数据展示

  • WB analysis using 29751-1-AP

    WB analysis using 29751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29751-1-AP (STUB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 29751-1-AP

    WB analysis of HeLa using 29751-1-AP

    WB result of STUB1 antibody (29751-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STUB1 transfected HeLa cells.

  • IHC staining of human placenta using 29751-1-AP

    IHC staining of human placenta using 29751-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STUB1 antibody included
  • IHC staining of human placenta using 29751-1-AP

    IHC staining of human placenta using 29751-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STUB1 antibody included
  • IHC staining of rat lung using 29751-1-AP

    IHC staining of rat lung using 29751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STUB1 antibody included
  • IHC staining of rat lung using 29751-1-AP

    IHC staining of rat lung using 29751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STUB1 antibody included
  • Featured Product
  • KD/KO验证

STUB1 Polyclonal antibody

STUB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29751-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CHIP, Antigen NY-CO-7, Carboxy terminus of Hsp70-interacting protein, CLL-associated antigen KW-8, E3 ubiquitin-protein ligase CHIP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, PC-12 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inrat lung tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STUB1 Ready-To-Use IHC Kit. STUB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29751-1-AP targets STUB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 STUB1 fusion protein Ag31335 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 STIP1 homology and U-box containing protein 1
别名 CHIP, Antigen NY-CO-7, Carboxy terminus of Hsp70-interacting protein, CLL-associated antigen KW-8, E3 ubiquitin-protein ligase CHIP
观测分子量35 kDa
GenBank蛋白编号BC007545
基因名称 STUB1
Gene ID (NCBI) 10273
RRIDAB_2923603
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNE7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STIP1 Homology and U-Box Containing Protein 1 (STUB1), formerly known as CHIP (C-terminus of HSP70-Interacting Protein), is a chaperone-dependent E3 ubiquitin ligase. STUB1 is a 34.5 kDa co-chaperone encoded by the STUB1 gene. STUB1 is highly conserved across species and is expressed in most tissues.

实验方案

Product Specific Protocols
WB protocol for STUB1 antibody 29751-1-APDownload protocol
IHC protocol for STUB1 antibody 29751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols