验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy STRN3 Ready-To-Use IHC Kit. STRN3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
23966-1-AP targets STRN3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag21091 Product name: Recombinant human STRN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 207-300 aa of BC126221 Sequence: SNSEPNGSVETKNLEQILNGGESPKQKGQEIKRSSGDVLETFNFLENADDSDEDEENDMIEGIPEGKDKHRMNKHKIGNEGLAADLTDDPDTEE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | striatin, calmodulin binding protein 3 |
| 别名 | Striatin-3, Striatin3, Striatin 3, SG2NA, PPP2R6B |
| 计算分子量 | 797 aa, 87 kDa |
| 观测分子量 | 90-100 kDa |
| GenBank蛋白编号 | BC126221 |
| 基因名称 | STRN3 |
| Gene ID (NCBI) | 29966 |
| RRID | AB_2879379 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q13033 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for STRN3 antibody 23966-1-AP | Download protocol |
| WB protocol for STRN3 antibody 23966-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Biol Cell Spatial proximity of proteins surrounding zyxin under force-bearing conditions. | ||
STAR Protoc Protocol using lentivirus to establish THP-1 suspension cell lines for immunostaining and confocal microscopy | ||
Genes Genomics LncRNA KTN1-AS1 facilitates esophageal squamous cell carcinoma progression via miR-885-5p/STRN3 axis | ||
Adv Sci (Weinh) TRAF3IP3 Induces ER Stress-Mediated Apoptosis with Protective Autophagy to Inhibit Lung Adenocarcinoma Proliferation |
