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验证数据展示

  • WB analysis using 66712-1-Ig (same clone as 66712-1-PBS)

    WB analysis using 66712-1-Ig (same clone as 66712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66712-1-Ig (STRAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66712-1-Ig (same clone as 66712-1-PBS)

    WB analysis of HEK-293 using 66712-1-Ig (same clone as 66712-1-PBS)

    WB result of STRAP antibody (66712-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STRAP transfected HEK-293 cells. This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 66712-1-Ig (same clone as 66712-1-PBS)

    IHC staining of mouse ovary using 66712-1-Ig (same clone as 66712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66712-1-Ig (STRAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STRAP antibody included
  • IHC staining of mouse ovary using 66712-1-Ig (same clone as 66712-1-PBS)

    IHC staining of mouse ovary using 66712-1-Ig (same clone as 66712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66712-1-Ig (STRAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STRAP antibody included
  • IF Staining of HEK-293 using 66712-1-Ig (same clone as 66712-1-PBS)

    IF Staining of HEK-293 using 66712-1-Ig (same clone as 66712-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STRAP antibody (66712-1-Ig, Clone: 5E11F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

STRAP Monoclonal antibody, PBS Only

STRAP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66712-1-PBS

产品说明书

CloneNo. 5E11F3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy STRAP Ready-To-Use IHC Kit. STRAP primary antibody included.

产品信息

66712-1-PBS targets STRAP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag13085

Product name: Recombinant human STRAP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC000162

Sequence: MAMRQTPLTCSGHTRPVVDLAFSGITPYGYFLISACKDGKPMLRQGDTGDWIGTFLGHKGAVWGATLNKDATKAATAAADFTAKVWDAVSGDELMTLAHKHIVKTVDFTQDSNYLLTGGQDKLLRIYDLNKPEAEPKEISGHTSGIKKALWCSEDKQILSADDKTVRLWDHATMTEVKSLNFNMSVSSMEYIPEGEILVITYGRSIAFHSAVSLDPIKSFEAPATINSASLHPEKEFLVAGGEDFKLYKYDYNSGEELESYKGHFGPIHCVRFSPDGELYASGSEDGTLRLWQTVVGKTYGLWKCVLPEEDSGELAKPKIGFPETTEEELEEIASENSDCIFPSAPDVKA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serine/threonine kinase receptor associated protein
别名 MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD
计算分子量 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC000162
基因名称 STRAP
Gene ID (NCBI) 11171
RRIDAB_2882063
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y3F4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.

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