验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, human liver tissue, human lung tissue, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12046-1-AP targets Stomatin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2676 Product name: Recombinant human STOM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-288 aa of BC010703 Sequence: MAEKRHTRDSEAQRLPDSFKDSPSKGLGPCGWILVAFSFLFTVITFPISIWMCIKIIKEYERAIIFRLGRILQGGAKGPGLFFILPCTDSFIKVDMRTISFDIPPQEILTKDSVTISVDGVVYYRVQNATLAVANITNADSATRLLAQTTLRNVLGTKNLSQILSDREEIAHNMQSTLDDATDAWGIKVERVEIKDVKLPVQLQRAMAAEAEASREARAKVIAAEGEMNASRALKEASMVITEYPAALQLRYLQTLTTIAAEKNSTIVFPLPIDMLQGIIGAKHSHLG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | stomatin |
| 别名 | STOM, BND7, EPB7, EPB72, Erythrocyte membrane protein band 7.2 |
| 计算分子量 | 288 aa, 32 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC010703 |
| 基因名称 | Stomatin |
| Gene ID (NCBI) | 2040 |
| RRID | AB_2197406 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P27105 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Stomatin antibody 12046-1-AP | Download protocol |
| IHC protocol for Stomatin antibody 12046-1-AP | Download protocol |
| IP protocol for Stomatin antibody 12046-1-AP | Download protocol |
| WB protocol for Stomatin antibody 12046-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Cancer Role of arachidonic acid metabolism in osteosarcoma prognosis by integrating WGCNA and bioinformatics analysis | ||
Front Med Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome | ||
Front Oncol Upregulation of stomatin is associated with poor prognosis and promotes tumor progression of orbital diffuse large B-cell lymphoma | ||
Vox Sang Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools. | ||
Chin J Cancer Res Stomatin plays a suppressor role in non-small cell lung cancer metastasis. | ||
Biophys J Interaction with stomatin directs human proton channels into cholesterol-dependent membrane domains |
