验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66152-1-Ig targets STMN3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag18844 Product name: Recombinant human STMN3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-180 aa of BC009381 Sequence: MASTISAYKEKMKELSVLSLICSCFYTQPHPNTVYQYGDMEVKQLDKRASGQSFEVILKSPSDLSPESPMLSSPPKKKDTSLEELQKRLEAAEERRKTQEAQVLKQLAERREHEREVLHKALEENNNFSRQAEEKLNYKMELSKEIREAHLAALRERLREKELHAAEVRRNKEQREEMSG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | stathmin-like 3 |
| 别名 | 1F12B1, SCG10-like protein, SCLIP, Stathmin 3, stathmin like 3 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC009381 |
| 基因名称 | STMN3 |
| Gene ID (NCBI) | 50861 |
| RRID | AB_2881548 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9NZ72 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Stathmin family phosphoproteins participate in the control of microtubule dynamics and have been proposed to be involved in the control of neuronal differentiation. Neuron specific Stathmin-3 (STMN3) is a novel STAT3 (signal transducer and activator of transcription 3) interacting protein. STAT3 is a key contributor to cancer cell migration and invasion. STMN3 exhibits microtubule-destabilizing activity, which is antagonized by STAT3. SCG10 is a widely studied and recognized neuronal differentiation marker, STMN3 is a SCG10-like protein (SCLIP) and is present from the earliest stages of hippocampal neuron differentiation in culture at vesicle-like structures following dynamic microtubules. RNAi mediated inhibition of this gene resulted in increased axonal branching.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STMN3 antibody 66152-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
