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验证数据展示

  • WB analysis using 82925-1-RR (same clone as 82925-1-PBS)

    WB analysis using 82925-1-RR (same clone as 82925-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82925-1-RR (STMN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 82925-1-RR (same clone as 82925-1-PBS)

    IP experiment of mouse brain using 82925-1-RR (same clone as 82925-1-PBS)

    IP result of anti-STMN2 (IP:82925-1-RR, 4ug; Detection:82925-1-RR 1:5000) with mouse brain tissue lysate 1800 ug. This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82925-1-RR (same clone as 82925-1-PBS)

    IHC staining of mouse brain using 82925-1-RR (same clone as 82925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-1-RR (STMN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STMN2 antibody included
  • IF Staining of mouse brain using 82925-1-RR (same clone as 82925-1-PBS)

    IF Staining of mouse brain using 82925-1-RR (same clone as 82925-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STMN2 antibody (82925-1-RR, Clone: 230110D5 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 82925-1-RR (same clone as 82925-1-PBS)

    IF Staining of SH-SY5Y using 82925-1-RR (same clone as 82925-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (82925-1-RR, Clone: 230110D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 82925-1-RR (same clone as 82925-1-PBS)

    FC experiment of SH-SY5Y using 82925-1-RR (same clone as 82925-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug STMN2 Recombinant antibody (82925-1-RR, Clone:230110D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82925-1-RR

    Affinity and Kinetic Characterization of 82925-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82925-1-RR against Human STMN2 were performed. The affinity constant is 1.29 nM.

STMN2 Recombinant monoclonal antibody, PBS Only

STMN2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA
Cat No. 82925-1-PBS

产品说明书

CloneNo. 230110D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

别名

230110D5, SCG 10, SCG10, SCGN 10, SCGN10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy STMN2 Ready-To-Use IHC Kit. STMN2 primary antibody included.

产品信息

82925-1-PBS targets STMN2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0929

Product name: Recombinant human STMN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-179 aa of BC006302

Sequence: MAKTAMAYKEKMKELSMLSLICSCFYPEPRNINIYTYDDMEVKQINKRASGQAFELILKPPSPISEAPRTLASPKKKDLSLEEIQKKLEAAEERRKSQEAQVLKQLAEKREHEREVLQKALEENNNFSKMAEEKLILKMEQIKENREANLAAIIERLQEKERHAAEVRRNKELQVELSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 stathmin-like 2
别名 230110D5, SCG 10, SCG10, SCGN 10, SCGN10
计算分子量 21 kDa
观测分子量 18-21 kDa
GenBank蛋白编号BC006302
基因名称 STMN2
Gene ID (NCBI) 11075
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ93045
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

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