STK36 Polyclonal antibody
STK36 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human prostate tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy STK36 Ready-To-Use IHC Kit. STK36 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12559-1-AP targets STK36 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, IP, WB,ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | STK36 fusion protein Ag3279 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | serine/threonine kinase 36, fused homolog (Drosophila) |
| Synonyms | FU, Fused homolog, KIAA1278, STK36 |
| Calculated Molecular Weight | 1315 aa, 144 kDa |
| Observed Molecular Weight | 144 kDa |
| GenBank Accession Number | BC026158 |
| Gene Symbol | STK36 |
| Gene ID (NCBI) | 27148 |
| RRID | AB_2196785 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRP7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STK36(Serine/threonine-protein kinase 36) is also named as KIAA1278 and belongs to the Ser/Thr protein kinase family. The Serine/threonine protein kinase plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors and controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STK36 antibody 12559-1-AP | Download protocol |
| IHC protocol for STK36 antibody 12559-1-AP | Download protocol |
| IP protocol for STK36 antibody 12559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Biol Mammalian Fused is essential for sperm head shaping and periaxonemal structure formation during spermatogenesis. | ||
FASEB J The putative protein kinase Stk36 is essential for ciliogenesis and CSF flow by associating with Ulk4 |