验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
16223-1-AP targets STK32A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag9276 Product name: Recombinant human STK32A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC021666 Sequence: MGANTSRKPPVFDENEDVNFDHFEILRAIGKGSFGKVCIVQKNDTKKMYAMKYMNKQKCVERNEVRNVFKELQIMQGLEHPFLVNLWYSFQDEEDMFMVVDLLLGGDLRYHLQQNVHFKEETVKLFICELVMALDYLQNQRIIHRDMKPDNILLDEHDTWLSYKSH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serine/threonine kinase 32A |
| 别名 | serine/threonine kinase 32A, STK32A, YANK1 |
| 计算分子量 | 396aa,46 kDa; 166aa,20 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC021666 |
| 基因名称 | STK32A |
| Gene ID (NCBI) | 202374 |
| RRID | AB_2196485 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WU08 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STK32A(Serine/threonine-protein kinase 32A) is also named as YANK1 and belongs to Ser/Thr protein kinase family. It can affect cell metabolism by protein serine/threonine kinase phosphorylation. The gene encoding STK32A maps to human chromosome 5 and this gene is associated with Cockayne syndrome through the ERCC8 gene and familial adenomatous polyposis through the adenomatous polyposis coli (APC) tumor suppressor gene.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STK32A antibody 16223-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
