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验证数据展示

  • WB analysis using 66637-1-Ig (same clone as 66637-1-PBS)

    WB analysis using 66637-1-Ig (same clone as 66637-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66637-1-Ig (STK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66637-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66637-1-Ig (same clone as 66637-1-PBS)

    WB analysis of HeLa using 66637-1-Ig (same clone as 66637-1-PBS)

    WB result of STK3 antibody (66637-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK3 transfected HeLa cells. This data was developed using the same antibody clone with 66637-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 66637-1-Ig (same clone as 66637-1-PBS)

    IF Staining of NIH/3T3 using 66637-1-Ig (same clone as 66637-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using STK3 antibody (66637-1-Ig, Clone: 1C11A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66637-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 66637-1-Ig (same clone as 66637-1-PBS)

    IHC staining of mouse kidney using 66637-1-Ig (same clone as 66637-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66637-1-Ig (STK3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66637-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STK3 antibody included
  • IHC staining of mouse kidney using 66637-1-Ig (same clone as 66637-1-PBS)

    IHC staining of mouse kidney using 66637-1-Ig (same clone as 66637-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66637-1-Ig (STK3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66637-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STK3 antibody included
  • Featured Product
  • KD/KO验证

STK3/MST2 Monoclonal antibody, PBS Only

STK3/MST2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66637-1-PBS

产品说明书

CloneNo. 1C11A4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MST2, STK3, 1C11A4, EC:2.7.11.1, KRS1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy STK3 Ready-To-Use IHC Kit. STK3 primary antibody included.

产品信息

66637-1-PBS targets STK3/MST2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2741

Product name: Recombinant human STK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-306 aa of BC010640

Sequence: MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQ

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 serine/threonine kinase 3 (STE20 homolog, yeast)
别名 MST2, STK3, 1C11A4, EC:2.7.11.1, KRS1
计算分子量 491 aa, 56 kDa
观测分子量 56-63 kDa
GenBank蛋白编号BC010640
基因名称 STK3
Gene ID (NCBI) 6788
RRIDAB_2881996
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13188
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The protein encoded by STK3 gene is a key serine/threonine kinase, and its protein product can be cleaved by caspase in the process of apoptosis, and the inhibitory C-terminal part is removed, and the N-terminal part is transferred to the nucleus to form homodimer, which becomes an active kinase and promotes chromatin condensation in the process of apoptosis. STK3 is one of the core components of Hippo signaling pathway, which plays a key role in controlling cell proliferation, apoptosis and tissue growth. In the Hippo signaling pathway, STK3 forms a cascade reaction with STK4, LATS1/2 and other kinases, phosphorylates and activates LATS1/2, and then inhibits transcription coactivators YAP and TAZ, thus controlling cell growth and preventing cancer progression. STK3 and STK4(MST1) are similar in structure and function. They have conserved amino-terminal kinase domains, carboxyl-terminal regulatory domains and interaction domains. The antibody can cross-recognize STK4.

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