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验证数据展示

  • WB analysis using 29846-1-AP

    WB analysis using 29846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29846-1-AP (STK24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29846-1-AP

    WB analysis using 29846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29846-1-AP (STK24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 29846-1-AP

    IHC staining of mouse testis using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 29846-1-AP

    IHC staining of mouse testis using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

STK24 Polyclonal antibody

STK24 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29846-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

MST 3, MST3, MST3B, STE20, STE20 like kinase MST3, STK24, STK3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse lung tissue, HeLa cells, Jurkat cells, rat lung tissue, rat skin tissue, rat spleen tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29846-1-AP targets STK24 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag31519

Product name: Recombinant human STK24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 320-443 aa of NM_003576

Sequence: SDAETDGQASGGSDSGDWIFTIREKDPKNLENGALQPSDLDRNKMKDIPKRPFSQCLSTIISPLFAELKEKSQACGGNLGSIEELRGAIYLAEEACPGISDTMVAQLVQRLQRYSLSGGGTSSH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine/threonine kinase 24 (STE20 homolog, yeast)
别名 MST 3, MST3, MST3B, STE20, STE20 like kinase MST3, STK24, STK3
计算分子量49KD
观测分子量50 kDa
GenBank蛋白编号NM_003576
基因名称 STK24
Gene ID (NCBI) 8428
RRIDAB_2923614
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6E0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serine/threonine-protein kinase 24 (STK24), also named MST3, belonging to GCK subfamily of STE20-like kinases, acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation (PubMed: 10644707, PMID:12107159). STK24 and STK25 operate in the same cardiovascular development pathway with programmed cell death 10 (CCM3) (PMID: 20332113). It regulates axon outgrowth in forebrain neurons (PubMed: 17114295).

实验方案

Product Specific Protocols
WB protocol for STK24 antibody 29846-1-APDownload protocol
IHC protocol for STK24 antibody 29846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols