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STK24 Polyclonal antibody

STK24 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29846-1-AP

Print datasheet

Synonyms

MST 3, MST3, MST3B, STE20, STE20 like kinase MST3, STK24, STK3

验证数据展示

  • WB analysis using 29846-1-AP

    WB analysis using 29846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29846-1-AP (STK24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29846-1-AP

    WB analysis using 29846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29846-1-AP (STK24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 29846-1-AP

    IHC staining of mouse testis using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 29846-1-AP

    IHC staining of mouse testis using 29846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29846-1-AP (STK24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse lung tissue, HeLa cells, Jurkat cells, rat lung tissue, rat skin tissue, rat spleen tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29846-1-AP targets STK24 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen STK24 fusion protein Ag31519 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine/threonine kinase 24 (STE20 homolog, yeast)
Synonyms MST 3, MST3, MST3B, STE20, STE20 like kinase MST3, STK24, STK3
Calculated Molecular Weight49KD
Observed Molecular Weight50 kDa
GenBank Accession NumberNM_003576
Gene Symbol STK24
Gene ID (NCBI) 8428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6E0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serine/threonine-protein kinase 24 (STK24), also named MST3, belonging to GCK subfamily of STE20-like kinases, acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation (PubMed: 10644707, PMID:12107159). STK24 and STK25 operate in the same cardiovascular development pathway with programmed cell death 10 (CCM3) (PMID: 20332113). It regulates axon outgrowth in forebrain neurons (PubMed: 17114295).

实验方案

Product Specific Protocols
WB protocol for STK24 antibody 29846-1-APDownload protocol
IHC protocol for STK24 antibody 29846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols