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验证数据展示

  • WB analysis of K-562 using 66189-1-Ig (same clone as 66189-1-PBS)

    WB analysis of K-562 using 66189-1-Ig (same clone as 66189-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

STIM1 Monoclonal antibody, PBS Only

STIM1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66189-1-PBS

产品说明书

CloneNo. 1D3A3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

STRMK, stim-1, STIM 1, IMD10, GOK

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66189-1-PBS targets STIM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17914

Product name: Recombinant human STIM1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 2-350 aa of BC021300

Sequence: DVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKW

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 stromal interaction molecule 1
别名 STRMK, stim-1, STIM 1, IMD10, GOK
计算分子量 685 aa, 77 kDa
观测分子量 77 kDa
GenBank蛋白编号BC021300
基因名称 STIM1
Gene ID (NCBI) 6786
RRIDAB_2881584
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13586
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.