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验证数据展示

  • WB analysis using 87526-1-RR

    WB analysis using 87526-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87526-1-RR (STBD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 87526-1-RR

    IF Staining of HeLa using 87526-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STBD1 antibody (87526-1-RR, Clone: 252915D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87526-1-RR

    Affinity of 87526-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87526-1-RR against Human STBD1 were performed. The affinity constant is 0.666 nM.

STBD1 Recombinant monoclonal antibody

STBD1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87526-1-RR

产品说明书

CloneNo. 252915D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Genethonin-1, GENX-3414, Glycophagy cargo receptor STBD1, starch binding domain 1, Starch-binding domain-containing protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, A549 cells, Caki-1 cells, HuH-7 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STBD1 Ready-To-Use IHC Kit. STBD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87526-1-RR targets STBD1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2481

Product name: Recombinant human STBD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 26-358 aa of BC022301

Sequence: GPGDTGKDGDAEQEKDAPLGGAAIPGGHQSGSSGLSPGPSGQELVTKPEHLQESNGHLISKTKDLGKLQAASWRLQNPSREVCDNSREHVPSGQFPDTEAPATSETSNSRSYSEVSRNESLESPMGEWGFQKGQEISAKAATCFAEKLPSSNLLKNRAKEEMSLSDLNSQDRVDHEEWEMVPRHSSWGDVGVGGSLKAPVLNLNQGMDNGRSTLVEARGQQVHGKMERVAVMPAGSQQVSVRFQVHYVTSTDVQFIAVTGDHECLGRWNTYIPLHYNKDGFWSHSIFLPADTVVEWKFVLVENGGVTRWEECSNRFLETGHEDKVVHAWWGIH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 starch binding domain 1
别名 Genethonin-1, GENX-3414, Glycophagy cargo receptor STBD1, starch binding domain 1, Starch-binding domain-containing protein 1
计算分子量 41 kDa
观测分子量 35-43 kDa
GenBank蛋白编号BC022301
基因名称 STBD1
Gene ID (NCBI) 8987
RRIDAB_3745598
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95210
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.

实验方案

Product Specific Protocols
IF protocol for STBD1 antibody 87526-1-RRDownload protocol
WB protocol for STBD1 antibody 87526-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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