验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, THP-1 cells, Jurkat cells, PC-12 cells |
| Positive IHC detected in | mouse lung tissue, human colon cancer tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy STAT6 Ready-To-Use IHC Kit. STAT6 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51073-1-AP targets STAT6 in WB, IHC, IF, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | signal transducer and activator of transcription 6, interleukin-4 induced |
| 别名 | IL-4 Stat, Signal transducer and activator of transcription 6, STAT 6, STAT6B, STAT6C |
| 计算分子量 | 94 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC075852 |
| 基因名称 | STAT6 |
| Gene ID (NCBI) | 6778 |
| RRID | AB_2197244 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42226 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. The antibody could identify about 100 kDa band, similar to the reported in the literature(PMID: 10961983).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for STAT6 antibody 51073-1-AP | Download protocol |
| WB protocol for STAT6 antibody 51073-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res Tumor-derived CCL16 Normalizes Tumor Vasculature through Macrophage ICAM-1 Receptor and Enhances Immunotherapy Efficacy in Hepatocellular Carcinoma | ||
Bone Res Nuclear FGF2 orchestrates phase separation-mediated rDNA chromatin architecture to control BMSCs cell fate | ||
Nat Commun Molecular landscape, subtypes, and therapeutic vulnerabilities of central nervous system solitary fibrous tumors | ||
Nat Commun Proteome-wide ligandability maps of drugs with diverse cysteine-reactive chemotypes | ||
Redox Biol USP10 protects against pressure overload-induced mitochondrial morphofunctional defects and pathological cardiac hypertrophy through stabilizing cytoplasmic Mfn2 | ||
Cell Death Dis Ciclopirox drives growth arrest and autophagic cell death through STAT3 in gastric cancer cells |
